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Kwigana imiterere ya physiologique ifasha abashakashatsi kubona ibyuma bifata ibyuma

Abashakashatsi bakoze uburyo bwo kumenya molekile nto ihuza ibyuma bya ion.Iyoni z'ibyuma ni ngombwa muri biologiya.Ariko kumenya molekile-cyane cyane molekile nto-izo ion zikorana birashobora kugorana.

Gutandukanya metabolite kugirango isesengurwe, uburyo busanzwe bwa metabolomics bukoresha imashanyarazi hamwe na pH nkeya, bishobora gutera ibyuma bitandukana.Pieter C. Dorrestein wo muri kaminuza ya Californiya San Diego hamwe n’abo bakorana bifuzaga guhuriza hamwe ibigo kugira ngo babisesengure bigana imiterere kavukire iboneka mu tugari.Ariko niba bakoresheje imiterere ya physiologique mugihe cyo gutandukanya molekile, bagombaga kongera guhindura uburyo bwo gutandukana kuri buri miterere yumubiri bashakaga kwipimisha.

Ahubwo, abashakashatsi bashizeho uburyo bubiri bwerekana uburyo bwa physiologique hagati yo gutandukana kwa chromatografiya no gusesengura ibintu rusange (Nat. Chem. 2021, DOI: 10.1038 / s41557-021-00803-1).Ubwa mbere, batandukanije ibinyabuzima bakoresheje chromatografiya isanzwe ikora cyane.Hanyuma bahinduye pH yumusozo usohoka muburyo bwa chromatografique kugirango bigane imiterere ya physiologique, bongeramo ion ibyuma, banasesengura imvange hamwe na sprometrike.Bakoze isesengura inshuro ebyiri kugirango babone spekrice ya molekile ntoya kandi idafite ibyuma.Kugirango umenye molekile zihuza ibyuma, bakoresheje uburyo bwo kubara bukoresha imiterere yimpinga kugirango berekane isano iri hagati yimiterere ihuza imipaka.

Dorrestein avuga ko bumwe mu buryo bwo kurushaho kwigana imiterere ya physiologiya, kwaba ari ukongeramo intungamubiri nyinshi za ion nka sodium cyangwa potasiyumu hamwe n’ubushyuhe buke bw’icyuma.Ati: “Biba igeragezwa ryo guhatanira.Bizakubwira ahanini, OK, iyi molekile muri ibyo bihe ifite amahirwe menshi yo guhambira sodium na potasiyumu cyangwa iki cyuma kidasanzwe wongeyeho ”, Dorrestein.“Turashobora gushiramo icyarimwe ibyuma byinshi bitandukanye icyarimwe, kandi dushobora kumva neza ibyifuzo no guhitamo muri urwo rwego.”

Mu mico yakuwe muri Escherichia coli, abashakashatsi bagaragaje ibintu bizwiho guhuza ibyuma nka yersiniabactin na aerobactin.Kubijyanye na yersiniabactin, bavumbuye ko ishobora no guhuza zinc.

Abashakashatsi bagaragaje ibyuma bifatanyiriza hamwe mu ngero zigoye nk'ibinyabuzima biva mu nyanja.Dorrestein agira ati: "Urwo rwose ni rumwe mu ngero zigoye cyane nigeze kureba."Ati: “Birashoboka ko bitoroshye nka peteroli itoroshye.”Uburyo bwerekanaga aside domoic nka molekile ihuza umuringa hanyuma isaba ko ihuza Cu2 + nka dimer.

Oliver Baars wiga metabolite ihuza ibyuma ikomoka ku bimera na mikorobe muri kaminuza ya Leta ya Carolina y'Amajyaruguru, yaranditse ati: imeri.

Muri email ye, Albert JR Heck, umupayiniya mu isesengura rusange ry’imyororokere muri kaminuza ya Utrecht, yanditse ati: “Dorrestein n'abo bakorana batanga uburyo bwiza, bukenewe cyane, kugira ngo basuzume neza uruhare rw'imiterere ya ioni y'icyuma mu kagari.”Ati: "Intambwe ikurikiraho ni ugukuramo metabolite mu bihe bya kavukire no kuyigabanyamo ibice no mu bihe bya kavukire, kugira ngo tumenye metabolite ikora ibyuma bya selile ya selile."

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