Amakuru

Kwigana imiterere ya physiologique ifasha abashakashatsi kubona ibyuma bihuza

Abashakashatsi bakoze uburyo bwo kumenya molekile ntoya ihuza ibyuma bya ion. Iyoni z'ibyuma ni ngombwa muri biologiya. Ariko kumenya molekile-cyane cyane molekile ntoya-izo ion zikorana nabyo birashobora kugorana.

Gutandukanya metabolite yo gusesengura, uburyo busanzwe bwa metabolomics bukoresha ibishishwa kama na pH nkeya, bishobora gutera ibyuma bitandukana. Pieter C. Dorrestein wo muri kaminuza ya Californiya San Diego na bagenzi be bifuzaga guhuriza hamwe ibigo kugira ngo babisesengure bigana imiterere kavukire iboneka mu tugari. Ariko baramutse bakoresheje imiterere ya physiologique mugihe cyo gutandukanya molekile, bagombaga kongera guhindura uburyo bwo gutandukana kuri buri miterere yumubiri bashakaga kwipimisha.

Ahubwo, abashakashatsi bashizeho uburyo bubiri bwerekana uburyo bwimiterere yimiterere hagati yo gutandukana kwa chromatografique hamwe nisesengura rusange (Nat. Chem. 2021, DOI: 10.1038 / s41557-021-00803-1). Ubwa mbere, batandukanije ibinyabuzima bakoresheje ibisanzwe byamazi meza ya chromatografiya. Noneho bahinduye pH yimigezi isohoka ya chromatografique kugirango bigane imiterere ya physiologique, bongeramo ion ibyuma, banasesengura imvange hamwe na sprometrike. Bakoze isesengura inshuro ebyiri kugirango babone sprake ya molekile ntoya kandi idafite ibyuma. Kugirango bamenye molekile zihuza ibyuma, bakoresheje uburyo bwo kubara bukoresha imiterere yimpinga kugirango bamenye isano iri hagati yimiterere ya verisiyo ihujwe.

Dorrestein avuga ko bumwe mu buryo bwo kurushaho kwigana imiterere y’imiterere y’imiterere, kwaba ari ukongera ingufu nyinshi za ion nka sodium cyangwa potasiyumu hamwe n’ubushyuhe buke bw’icyuma. Ati: “Biba igeragezwa ryo guhatana. Bizakubwira ahanini, Nibyo, iyi molekile muri ibyo bihe ifite amahirwe menshi yo guhuza sodium na potasiyumu cyangwa iki cyuma kidasanzwe wongeyeho ”, Dorrestein. Ati: "Turashobora gushiramo icyarimwe ibyuma byinshi bitandukanye, kandi dushobora kumva neza ibyifuzo no guhitamo muri urwo rwego."

Mu muco wakuwe muri Escherichia coli, abashakashatsi bagaragaje ibice bizwiho guhuza ibyuma nka yersiniabactin na aerobactin. Ku bijyanye na yersiniabactin, bavumbuye ko ishobora no guhambira zinc.

Abashakashatsi bagaragaje ibyuma bihuza ibyuma mu ngero zigoye nk'ibinyabuzima byashonze biva mu nyanja. Dorrestein agira ati: "Urwo rwose ni rumwe mu ngero zigoye cyane nigeze kureba." Ati: "Birashoboka ko bitoroshye, niba bitoroshye kuruta amavuta ya peteroli." Uburyo bwerekanaga aside domoic nka molekile ihuza umuringa kandi isaba ko ihuza Cu2 + nka dimer.

Oliver Baars wiga metabolite ihuza ibyuma ikorwa n'ibimera na mikorobe muri kaminuza ya Leta ya Carolina y'Amajyaruguru yaranditse ati: imeri.

Muri email ye, Albert JR Heck, umupayiniya mu isesengura rusange ry’imyororokere ya kaminuza muri kaminuza ya Utrecht, yanditse ati: “Dorrestein n'abo bakorana batanga uburyo bwiza, bukenewe cyane, kugira ngo basuzume neza uruhare rw'imiterere ya ioni y'ibyuma mu kagari.” Ati: “Intambwe ikurikiraho ni ugukuramo metabolite mu bihe bya kavukire no kuyigabanyamo ibice no mu bihe kavukire, kugira ngo tumenye metabolite itwara ibyuma bya selile selile.”

Igihe cyo kohereza: Ukuboza-23-2021